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Gene stacking as a strategy to confer characteristics of agronomic importance in plants by genetic engineering
Provedor de dados: Ciência Rural
Autores: Ceccon,Cássia Canzi; Caverzan,Andréia; Margis,Rogerio; Salvadori,José Roberto; Grando,Magali Ferrari.
ABSTRACT: Gene stacking refers to the introduction of two or more transgenes of agronomic interest in the same plant. The main methods for genetically engineering plants with gene stacking involve (i) the simultaneous introduction, by the co-transformation process, and (ii) the sequential introduction of genes using the re-transformation processes or the sexual crossing between separate transgenic events. In general, the choice of the best method varies according to the species of interest and the availability of genetic constructions and preexisting transgenic events. We also present here the use of minichromosome technology as a potential future gene stacking technology. The purpose of this review was to discuss aspects related to the methodology for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Co-transformation; Re-transformation; Sexual crossing; Transgene; Minichromosomes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000600401